Creatinine determined by "high-performance" liquid chromatography.
نویسندگان
چکیده
منابع مشابه
Creatinine determined by "high-performance" liquid chromatography.
We describe a "high-performance" liquid-chromatographic procedure for determination of creatinine in serum. Creatinine is separated from other species by cation-exchange chromatography. Absorbance of the creatinine in the eluate is monitored at 234 nm. Results from this procedure on sera correspond closely to those of a manual Jaffé-reaction procedure involving use of a protein-free filtrate wi...
متن کاملSEPARATION OF NONHISTONE HIGH MOBILITY GROUP (HMG) FROM HUMAN LYMPHOCYTES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...
متن کاملMicromethod for determination of creatinine in biological fluids by high-performance liquid chromatography.
We describe a procedure for the rapid and specific measurement of creatinine, in which it is separated from other compounds in serum or urine by paired-ion chromatography and is quantified by measuring its absorbance at 200 nm. The procedure can be done on as little as 10 microliter of serum. Between-day precision studies for concentrations of 13 and 62 mg/liter yielded coefficients of variatio...
متن کاملSerum creatinine determination by high performance liquid chromatography and five automated chemistry analyzers.
A sensitive and specific procedure is described for the determination of serum creatinine by reverse phase high performance liquid chromatography. The method involves sample pretreatment with a cation exchange resin followed by the isocratic separation of creatinine and an external standard which are detected by their absorbances at 254 nm. The analytical recovery of creatinine from serum was 9...
متن کاملSimultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography.
An environmentally friendly reversed-phase HPLC method for simultaneous determination of creatinine and uric acid in human urine samples has been developed. Human urine samples were pretreated by dilution, protein precipitation, centrifugation and filtration, followed by HPLC separations using a reversed-phase C(18) column with an aqueous mobile phase of phosphate buffer. The retention loss of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1983
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/29.2.256